Research on the antioxidant activity of metabolites from a sponge-derived fungus Alternaria sp. F49 / 药学学报

To study the chemical constituents from the the deep-sea fungus Alternaria sp. F49. Seven compounds were isolated from the EtOAc extract by using silica gel, Sephadex LH-20, ODS and HPLC methods. Based on the spectroscopic analysis, their structures were identified as (8R)-5-O-methyl-orcinotriol (1), orcinotriol (2), α-acetylorcinol (3), 3'-hydroxyalternariol 5-O-methyl ether (4), altenusiol (5), altenusin (6), and 5'-methoxy-6-methyl-biphenyl-3,4,3'-triol (7). (8R)-5-O-Methyl-orcinotriol (1) is a new phenolic compound which has never been reported in the literature. Compounds 4-7 showed strong DPPH free radical scavenging activity; whereas compounds 1-7 showed strong ABTS free radical scavenging activity.

Quality Evaluation of Brown Rice Sulgidduk Containing Acorn Powder

This study examined the quality characteristics of brown rice Sulgidduk containing acorn powder and the optimal mixing rate (0%, 5%, 10%, and 15%). The moisture contents of brown rice Sulgidduk increased with increasing amount of acorn powder. The DPPH free radical scavenging activities (16.15%~28.06%) and ABTS free radical scavenging activities (22.98%~42.81%) of the brown rice Sulgidduk increased with increasing acorn powder content. The L value of the Hunter's color value decreased with increasing amount of acorn powder. The hardness and chewiness of brown rice Sulgidduk increased with increasing amount of acorn powder. The brown rice Sulgidduk containing 10% acorn powder showed the highest score with regard to the sensory characteristics. These results suggest that the most desirable amount of acorn powder is 10% and the addition of acorn powder could contribute positively toward the quality characteristics of brown rice Sulgidduk.

Spectrum-effect relationship between HPLC fingerprint and free radicals scavenging in Guizhi Shaoyao Zhimu Decoction / 中国中药杂志

To establish the spectrum-effect relationship between HPLC fingerprint and free radicals activity scavenging in Guizhi Shaoyao Zhimu Decoction( GSZD),and provide a basis for the quality evaluation and modernization of classical prescriptions. Shimadsu GL-science C18 column( 4. 6 mm×250 mm,5 μm) was used with acetonitrile-0. 1% formic acid solution as the mobile phase for gradient elution. The detective wave length was 254 nm; the column temperature was set at 32 ℃; the injection volume was 20 μL; and the flow rate was 1. 0 m L·min-1.10 batches of primary standard samples of GSZD were detected,and their HPLC fingerprint was established by using the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( TCM). The activity of scavenging free radicals was studied by 1,1-diphenyl-2-trinitrophenylhydrazine( DPPH) method,and the spectrum-effect relationship was studied by Pearson bivariate correlation analysis. The common mode of GSZD fingerprints was established,and 26 common peaks were marked,with similarities ranging from 0. 929 to 0. 998. Eight of the chromatographic peaks were identified by using the control comparison method: gallic acid,mangiferin,paeoniflorin,glycyrrhizin,asparagus,5-O-methylvisamicin,cinnamic acid,and ammonium glycyrrhetate. Among them,the content changes of No. 14( paeoniside),20,12( mangiferin),13 and 23( cinnamic acid) common peaks were negatively correlated with free radical scavenging activity. The fingerprint showed high precision,repeatability and stability,and the common peaks were well separated,so it can be used for the quality evaluation of GSZD,and could provide reference for further studies on the material basis of GSZD.

Screening of Antioxidant Active Components of Schisandra chinensis / 中国药房

OBJECTIVE:To screen antioxidant active components of Schisandra chinensis. METHODS:The orthogonal test was adopted to optimize extraction technology using DPPH free radical scavenging activity(IC50)as index and ethanol volume frac-tion,material-liquid ratio and extraction time as factors,and the verification test were made. The fractions(SC-0,SC-10,SC-30, SC-50,SC-70,SC-95) were made by extracting and purifying S. chinensis with macroporous resin with water and 10%,30%, 50%,70%and 95%ethanol. With IC50 and total antioxidant capacity(determined by ABTS method)as indexes(vitamin C as pos-itive control),the antioxidant active components of S. chinensis were optimized. The contents of 5 kinds of lignan in different posi-tions of S. chinensis were determined by HPLC. RESULTS:The optimal extraction condition of S. chinensis was as follows as 60% ethanol,material-liquid ratio of 1∶14,extracting for 2.0 h. The average IC50 of DPPH free radical scavenging activity was 23.81 mg/ml(RSD=0.52%,n=3)in verification test. SC-0 did not have antioxidant abilities. DPPH free radical scavenging activi-ty of those components (ie. the IC50 value from low to high) were in the following order of positive control>SC-50>SC-30>SC-95>SC-70>SC-10;total antioxidant ability of them were in the following order of SC-50>positive control>SC-30>SC-70>SC-95>SC-10;the contents of 5 types of lignan in different components were in the following order of SC-70>SC-50>SC-95>SC-30. CONCLUSIONS:The antioxidant active component of S. chinensis is 50%ethanol eluate.

Evaluation of cytotoxic and antioxidant activity of different fractions of methanolic extract of Baccaurea ramiflora (Lour.) fruits.

Chloroform and petroleum ether soluble fractions of the crude methanolic extract of the fruits of Baccaurea ramiflora (Lour.) have been investigated for the evaluation of cytotoxic and antioxidant activity. Brine shrimp lethality bioassay was used to evaluate the cytotoxic activity and free radical scavenging activity method was used to evaluate antioxidant activity. In brine shrimp lethality bioassay, the chloroform soluble fraction showed greater cytotoxic activity (LC50 value 74.2 μg/ml) than petroleum ether soluble fraction (LC50 value 129 μg/ml) compared to the standard drug vincristine sulfate (LC50 value 0.54 μg/ml). In free radical scavenging activity method (DPPH assay method), the chloroform soluble fraction showed greater antioxidant activity (IC50 value 49.78 μg/ml) than petroleum ether soluble fraction (IC50 value 75.31 μg/ml) compared to the standard antioxidant butylated hydroxytoluene (IC50 value 24.51μg/ml). This study reveals that Baccaurea ramiflora fruit possesses cytotoxic and antioxidant activity hence can be a potential source for the isolation of active principle(s) for different therapies.

Evaluation of cytotoxic and antioxidant activity of different fractions of methanolic extract of Baccaurea ramiflora (Lour.) fruits.

Chloroform and petroleum ether soluble fractions of the crude methanolic extract of the fruits of Baccaurea ramiflora (Lour.) have been investigated for the evaluation of cytotoxic and antioxidant activity. Brine shrimp lethality bioassay was used to evaluate the cytotoxic activity and free radical scavenging activity method was used to evaluate antioxidant activity. In brine shrimp lethality bioassay, the chloroform soluble fraction showed greater cytotoxic activity (LC50 value 74.2 μg/ml) than petroleum ether soluble fraction (LC50 value 129 μg/ml) compared to the standard drug vincristine sulfate (LC50 value 0.54 μg/ml). In free radical scavenging activity method (DPPH assay method), the chloroform soluble fraction showed greater antioxidant activity (IC50 value 49.78 μg/ml) than petroleum ether soluble fraction (IC50 value 75.31 μg/ml) compared to the standard antioxidant butylated hydroxytoluene (IC50 value 24.51μg/ml). This study reveals that Baccaurea ramiflora fruit possesses cytotoxic and antioxidant activity hence can be a potential source for the isolation of active principle(s) for different therapies.

Anti-inflammatory and antioxidant activities of ethanolic extract of aerial parts of Vernonia patula (Dryand.) Merr

Objective:To investigate the inflammatory and antioxidant activities of ethanolic extract of aerial part of Vernonia patula (Dryand.) Merr (EAV). Methods:The anti-inflammatory activity of EAV was studied using carrageenan and histamine-induced rat paw edema test at different doses (100, 200 and 400 mg/kg body weight). DPPH free radical scavenging, nitric oxide scavenging, reducing power and Fe2+ion chelating ability were used for determining antioxidant activities. Results: The EAV, at the dose of 400 mg/kg, showed a significant anti-inflammatory activity (P Conclusions: Therefore, the obtained results suggest the acute anti-inflammatory and antioxidant activities of the EAV and thus provide the scientific basis for the traditional uses of this plant part as a remedy for inflammations.

Capacidade antioxidante de frutas / Antioxidant capacity of the fruits

Extratos aquoso e acetônico de 15 frutas foram submetidos a ensaios para investigar a habilidade de seqüestrar o radical estável 1,1-difenil-2-picrilhidrazil (DPPH) e a capacidade de inibir a oxidação em sistema modelo β-caroteno/ácido linoléico. Todas as frutas exibiram propriedade antioxidante, entretanto a ação foi diferenciada entre elas. O extrato aquoso da acerola, caju, mamão "Formosa", mamão Havaí, laranja pêra e goiaba foram os mais eficazes (superior a 70 por cento), enquanto que o do abacaxi, laranja cravo, manga rosa, melão espanhol, melão japonês, melão orange flesh e pinha apresentaram ação moderada (60-70 por cento) e o da manga espada e melancia exibiram a mais fraca capacidade de seqüestrar o radical DPPH. Os extratos acetônico da acerola, caju, pinha e goiaba exibiram uma forte capacidade de seqüestrar o radical DPPH (superior a 70 por cento). Em sistema modelo β-caroteno/ácido linoléico, o extrato aquoso da pinha e o acetônico da goiaba exibiram moderada capacidade antioxidante (60-70 por cento) enquanto que a acerola (extrato aquoso) e o mamão formosa (extrato acetônico) os menores percentuais. Frente à capacidade antioxidante exibida, as frutas podem ser apontadas como fontes de antioxidantes naturais, destacando-se a acerola, caju, mamão Formosa, mamão Havaí, goiaba, laranja pêra, e a pinha por terem apresentado uma potente capacidade antioxidante.

Aqueous and acetone extracts from 15 fruits have been screened for antioxidant activity using DPPH method and ß-carotene/linoleic acid model. All fruits studied showed antioxidant activity, but in different extent. Acerola, cashew-apple, papaya "formosa", papaya "solo", orange and guava showed the higher antioxidant activity (>70 percent) in DPPH method, followed by pineapple, bergamont, mango "rosa", melon "reticulares", melon "inodorus", melon "orange flesh" and sugar-apple aqueous extract (moderate, 60-70 percent) and mango "espada" and watermelon aqueous extract, the lowest activity. Acerola, cashew-apple, sugar-apple and guava acetone extracts exhibited higher scavenging activity toward DPPH radicals (>70 percent).β-carotene/linoleic acid model index of sugar-apple aqueous extract and guava acetone extract demonstrated moderate antioxidant activity (60-70 percent), meanwhile acerola aqueous extract and papaya "formosa" acetone extract the lower. According to antioxidant activity, fruits can be indicated as natural antioxidants sources, pointing out acerola, cashew-apple, papaya "solo", papaya "formosa", guava, orange and sugar-apple as the highest in antioxidant capacity.